Anti-GABA A Receptor gamma 2/GABRG2 Antibody Picoband™

Product Name

Anti-GABA A Receptor gamma 2/GABRG2 Antibody Picoband™

View all GABA-A R gamma 2 Antibodies

SKU/Catalog Number

A02361-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GABA A Receptor gamma 2/GABRG2 Antibody Picoband™ catalog # A02361-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GABA A Receptor gamma 2/GABRG2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02361-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human GABA A Receptor gamma 2/GABRG2 recombinant protein (Position: Q40-R446).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A02361-1 is reactive to GABRG2 in Human, Mouse, Rat

Applications

A02361-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

54.162kDa

Background of GABA-A R gamma 2

Gamma-aminobutyric acid receptor subunit gamma-2 is a protein that in humans is encoded by the GABRG2 gene. This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures. Multiple transcript variants encoding different isoforms have been identified for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse
Flow Cytometry, 1-3μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of GABA A Receptor gamma 2/GABRG2 using anti-GABA A Receptor gamma 2/GABRG2 antibody (A02361-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABA A Receptor gamma 2/GABRG2 antigen affinity purified polyclonal antibody (Catalog # A02361-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABA A Receptor gamma 2/GABRG2 at approximately 50KD. The expected band size for GABA A Receptor gamma 2/GABRG2 is at 50KD.

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Figure 2. IHC analysis of GABA A Receptor gamma 2/GABRG2 using anti-GABA A Receptor gamma 2/GABRG2 antibody (A02361-1).
GABA A Receptor gamma 2/GABRG2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GABA A Receptor gamma 2/GABRG2 Antibody (A02361-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 3. Flow Cytometry analysis of THP-1 cells using anti-GABA A Receptor gamma 2/GABRG2 antibody (A02361-1).
Overlay histogram showing THP-1 cells stained with A02361-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GABA A Receptor gamma 2/GABRG2 Antibody (A02361-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For GABRG2 (Source: Uniprot.org, NCBI)

Alternative Names

CAE2; ECA2; GABA A R gamma 2; GABA(A) receptor subunit gamma-2; GABAAR gamma 2; GABAARg2; GABRG2; gamma-aminobutyric acid (GABA) A receptor, gamma 2; gamma-aminobutyric acid receptor subunit gamma-2; GEFSP3 GABRG2 CAE2, DEE74, ECA2, EIEE74, FEB8, GEFSP3 gamma-aminobutyric acid type A receptor subunit gamma2 gamma-aminobutyric acid receptor subunit gamma-2|GABA(A) receptor subunit gamma-2|GABA(A) receptor, gamma 2|gamma-aminobutyric acid (GABA) A receptor, gamma 2|gamma-aminobutyric acid type A receptor gamma2 subunit

For more info on GABRG2, check out the GABRG2 Infographic

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In this infographic, you will see the following information for GABRG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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